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KMID : 0903519600010010055
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1960 Volume.1 No. 1 p.55 ~ p.61
Chromatographic Determination of Amino Acids in Nonprotein and Protein Fraction Of Undaria Pinnatifida


Abstract
The amino acid compositions of the protein and the nonprotein fractions obtained from a marine brown alga:, $quot;Undaria pinnatifida$quot; were determined by use of Ion Exchange Column Chromatography.
The protein nitrogen in the alga was about ten times of the nonprotein nitrogen. Nonprotein fraction obtained from the extraction with 80 per cent ethanol contains considerable amount of tree citrulline. Alanine content in the alga was the highest (about 1 per cent in dry weight) and one third of which was found in free state. The amino acid composition of the alga was well balanced and the content of the essential amino acids were relatively higher, than soybean protein.
In addition, several peptide like substances were fractionated from nonproatein fraction, in which one way identified as a naturally occurring new tripeptide composed of alanine, glutamic acid and aspartic acid, and the remaining unknown substances are under investigation for the further information.
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